Structural and kinetic analysis of helicase complexes in DNA mismatch repair

Recruitment for this position has closed.

PhD project: In this project the MMR helicase complex will be studied using ensemble and single molecule fluorescence approaches in order to determine its assembly, stoichiometry and activity. Objectives are to use multicolour fluorescence and FRET on protein and/or DNA to determine the presence and amount of proteins during different stages of the repair reactions, to analyse the timing of events and to follow the mechanistic details and structural changes in protein and DNA during the initiation of unwinding. Finally, to analyse the interaction between MMR and replication machinery in real time using fluorescently labelled proteins.

You will be immersed in an international, multidisciplinary training environment in fundamental life sciences and obtain insight into the role of basic chemical and physical principles underlying molecular steps in complex yet integrated molecular processes. As the research relies on cutting-edge technologies, development of new approaches will be an integral part of your project. Because of the close collaboration between academia and industry, you will furthermore learn how technology development drives innovation in research and how basic research questions can drive innovative technology into marketplace developments.

This project will be hosted at the Institute of Biochemistry, Department of Biology and Chemistry of Justus Liebig University (Giessen, Germany), with long and short training visits to other consortium partners.

Qualifications and skills

You have an MSc in in Biochemistry, Chemistry, Molecular Biology closely related field, good command of English (IELTS grade min. 6.5, or equivalent). Team working, communication skills and interdisciplinary work is prerequisite. Due to the basic idea of a Marie Curie ITN candidates should be highly mobile and able to spend several months' long periods in other institutions.

The Justus Liebig University aims to employ more women in academic research. We therefore particularly encourage female candidates to apply.

The Justus Liebig University aims to position itself as a family-friendly university. Applicants with children are welcome to apply.

Terms of employment

The successful candidate will receive a temporary position for 3 years. Payment will be according to EU Marie Curie Training Network regulations (

Contact information

For more information contact apl. Prof. Dr. Peter Friedhoff, Institute for Biochemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany,, Telephone ++49-641-9935407. Information on the Institute can be found at

Eligibility criteria and instructions on how to apply are listed on our vacancies page.
Application deadline for this position is March 15, 2017.