Structural analysis of relevant complexes in DNA mismatch repair

Recruitment for this position has closed.

PhD project: This project will address how conformational changes drive the process of DNA mismatch repair. You will use cryo-electron microscopy and protein crystallography to trap defined states in this process and to analyze how the proteins are activated. We will validate structures by different biophysical and biochemical approaches, testing for protein interaction, binding, enzyme activity and conformational change. You will be trained in protein purification and characterization, structural biology, biophysics and biochemistry to study the coordinated enzyme activities during DNA mismatch repair.

This project will be hosted at the Netherlands Cancer Institute (NKI) in Amsterdam, the Netherlands, with secondments and training visits to other consortium partners. The atmosphere in the Netherlands Cancer Institute is highly interactive, with multiple inter-group work discussion weekly and several informal fora. The structural biology groups share lab space and work discussions, providing high level expertise in protein methods, biophysics and protein crystallography. Graduate students participate in the OOA graduate school, allowing them to build a good local network and promoting interaction with other groups active in DNA repair and its effects for cancer.

Qualifications and skills

We are looking for an enthusiastic candidate with a MSc in Biochemistry, Structural Biology or closely related field, good command of English (IELTS grade min. 6.5, or equivalent). We welcome applicants of all nationalities. Being able to present a certificate of good conduct is a condition for the appointment.

Terms of employment

You will receive a temporary position for 4 years. The terms of employment are according to the Collective Bargaining Agreement for Hospitals (CAO Ziekenhuizen). Additionally, employment terms for Marie Curie projects ( apply for the first three years of employment.

Contact information

For more information on the DNA Repairman training network, see For more information about this position, please contact Prof. Dr. T.K. Sixma (

Eligibility criteria and instructions on how to apply are listed on our vacancies page.
Application deadline for this position is Friday February 10th, 2017.